Review




Structured Review

Molecular Instruments amplification buffer
Amplification Buffer, supplied by Molecular Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/amplification+buffer/amplification+buffer/10__1096_slash_fj__202601339r-65-10-14
Average 86 stars, based on 1 article reviews
amplification buffer - by Bioz Stars, 2026-09
86/100 stars

Images

Related Articles

Incubation:

Article Title: Cis-regulatory evolution shapes facial diversity in birds and mammals
Article Snippet: After the hybridization step, a series of four washes with wash buffer (Molecular Instruments Inc.) was performed to eliminate unbonded probes. .. Subsequently, embryos were incubated in a solution containing 30 pmol of hairpins H1 and H2 diluted in an amplification buffer (Molecular Instruments Inc.) overnight at room temperature, protected from light. .. After a wash in 0.1% 5×-SSC-Tween (20× SSC; Fisher Bioreagents, BP1325-4), embryos were incubated in 1× DAPI (4′,6-diamidino-2-phenylindole) solution (Thermo Fisher Scientific, D21490 ) overnight at 4°C on slow rotation.

Article Title: Hypoxia differentially affects coronary vessel formation during heart development.
Article Snippet: Excess probes were then removed by washing 6 in Probe Wash Buffer (Molecular Instruments) at 37°C, followed by washes in SSCT at room 7 temperature. .. Hearts were incubated in Amplification buffer (Molecular Instruments), whilst 8 Hairpin solution was prepared by adding snap-cooled h1 and h2 hairpins to Amplification 9 buffer according to manufacturer’s instructions. .. Hearts were incubated overnight in the 10 Hairpin solution in the dark, before washing in SSCT solution and imaging using a Zeiss 710 11 MP confocal microscope.

Article Title: Lysosome-related organelles orchestrate guanine crystal formation in pigment cells.
Article Snippet: Iridosomes, the guanine crystalforming organelles of pigmentproducing iridophores, are among the most versatile, visually striking yet mechanistically uncharacterized organelles in vertebrate biology.. Lysosomerelated organelles (LROs) support cell type–specific functions by adapting endolysosomal pathways for specialized roles.. Here, we show that iridosomes represent a subtype of LROs.

Article Title: Nr2f1a and Isl1 repress acquisition of epicardial identity in venous atrial cardiomyocytes.
Article Snippet: Embryos were first washed 4x in prewarmed probe wash buffer (Molecular Instruments) for 15 minutes each at 37°C followed by 2x with 5x Sodium chloride/sodium citrate/0.1% Tween (SSCT) at RT. .. Samples were incubated for 1 hour at RT in 500 μL of amplification buffer (Molecular instruments). ..

Article Title: A transcriptional atlas of early Arabidopsis seed development suggests mechanisms for inter-tissue coordination
Article Snippet: Probe washes closely followed the Molecular Instruments HCR protocol: we performed four 15-min buffer exchanges with Probe Wash Buffer (Molecular Instruments) preheated to 37 °C in a water bath, followed by two quick washes with 5× SSC-Tween-20. .. Samples in both positive and negative control tubes were incubated in Amplification Buffer (Molecular Instruments) for 30 min at room temperature before exchange to Amplification Buffer containing snap-cooled hairpins (8 μl of 3 μM stock per hairpin per 400 μl reaction volume). .. We used hairpins containing B2 and B3 adapter sequences with Alexa-488 or Alexa-647 dyes (Molecular Instruments).

Article Title: Elevated phagocytic capacity directs innate spinal cord repair.
Article Snippet: Washes were performed in wash buffer (Molecular Instruments) at 37◦C followed by 5× SSCT (3M NaCl, 0.3 M Sodium Citrate, 0.1% Tween 20, pH 7.0) at room temperature. .. For pre-amplification, sections were incubated in amplification buffer (Molecular Instruments) for 1 h at room temperature. ..

Amplification:

Article Title: Cis-regulatory evolution shapes facial diversity in birds and mammals
Article Snippet: After the hybridization step, a series of four washes with wash buffer (Molecular Instruments Inc.) was performed to eliminate unbonded probes. .. Subsequently, embryos were incubated in a solution containing 30 pmol of hairpins H1 and H2 diluted in an amplification buffer (Molecular Instruments Inc.) overnight at room temperature, protected from light. .. After a wash in 0.1% 5×-SSC-Tween (20× SSC; Fisher Bioreagents, BP1325-4), embryos were incubated in 1× DAPI (4′,6-diamidino-2-phenylindole) solution (Thermo Fisher Scientific, D21490 ) overnight at 4°C on slow rotation.

Article Title: Hypoxia differentially affects coronary vessel formation during heart development.
Article Snippet: Excess probes were then removed by washing 6 in Probe Wash Buffer (Molecular Instruments) at 37°C, followed by washes in SSCT at room 7 temperature. .. Hearts were incubated in Amplification buffer (Molecular Instruments), whilst 8 Hairpin solution was prepared by adding snap-cooled h1 and h2 hairpins to Amplification 9 buffer according to manufacturer’s instructions. .. Hearts were incubated overnight in the 10 Hairpin solution in the dark, before washing in SSCT solution and imaging using a Zeiss 710 11 MP confocal microscope.

Article Title: Multiscale regulation of experience-dependent plasticity by a Pannexin1 homolog in a developing vertebrate brain
Article Snippet: To remove excess probes, the samples were washed 4 times for 15 mins each with a wash buffer (Molecular Instruments) at 37°C, followed by 2 washes of 5 mins each with 5× SSCT (5× SSC + 0.1% Tween-20) at RT. .. Pre-amplification was performed by incubating the samples for 30 mins in an amplification buffer (Molecular Instruments) at RT. ..

Article Title: Lysosome-related organelles orchestrate guanine crystal formation in pigment cells.
Article Snippet: Iridosomes, the guanine crystalforming organelles of pigmentproducing iridophores, are among the most versatile, visually striking yet mechanistically uncharacterized organelles in vertebrate biology.. Lysosomerelated organelles (LROs) support cell type–specific functions by adapting endolysosomal pathways for specialized roles.. Here, we show that iridosomes represent a subtype of LROs.

Article Title: Nr2f1a and Isl1 repress acquisition of epicardial identity in venous atrial cardiomyocytes.
Article Snippet: Embryos were first washed 4x in prewarmed probe wash buffer (Molecular Instruments) for 15 minutes each at 37°C followed by 2x with 5x Sodium chloride/sodium citrate/0.1% Tween (SSCT) at RT. .. Samples were incubated for 1 hour at RT in 500 μL of amplification buffer (Molecular instruments). ..

Article Title: A transcriptional atlas of early Arabidopsis seed development suggests mechanisms for inter-tissue coordination
Article Snippet: Probe washes closely followed the Molecular Instruments HCR protocol: we performed four 15-min buffer exchanges with Probe Wash Buffer (Molecular Instruments) preheated to 37 °C in a water bath, followed by two quick washes with 5× SSC-Tween-20. .. Samples in both positive and negative control tubes were incubated in Amplification Buffer (Molecular Instruments) for 30 min at room temperature before exchange to Amplification Buffer containing snap-cooled hairpins (8 μl of 3 μM stock per hairpin per 400 μl reaction volume). .. We used hairpins containing B2 and B3 adapter sequences with Alexa-488 or Alexa-647 dyes (Molecular Instruments).

Article Title: Retinoic Acid Informs the Positional Identity of Frontonasal Neural Crest Cells Through Alx Family of Transcription Factors
Article Snippet: After hybridization, samples were washed four times using HCR washing buffer (HCR Buffers, Molecular Instruments, USA) at 37°C, followed by two washes in 5xSSCT at RT. .. Next, after 5 min of pre- amplification in 500 μL amplification buffer (HCR Buffers, Molecular Instruments, USA), the embryos were kept in amplification solution overnight in the dark at RT. ..

Article Title: Elevated phagocytic capacity directs innate spinal cord repair.
Article Snippet: Washes were performed in wash buffer (Molecular Instruments) at 37◦C followed by 5× SSCT (3M NaCl, 0.3 M Sodium Citrate, 0.1% Tween 20, pH 7.0) at room temperature. .. For pre-amplification, sections were incubated in amplification buffer (Molecular Instruments) for 1 h at room temperature. ..

Negative Control:

Article Title: A transcriptional atlas of early Arabidopsis seed development suggests mechanisms for inter-tissue coordination
Article Snippet: Probe washes closely followed the Molecular Instruments HCR protocol: we performed four 15-min buffer exchanges with Probe Wash Buffer (Molecular Instruments) preheated to 37 °C in a water bath, followed by two quick washes with 5× SSC-Tween-20. .. Samples in both positive and negative control tubes were incubated in Amplification Buffer (Molecular Instruments) for 30 min at room temperature before exchange to Amplification Buffer containing snap-cooled hairpins (8 μl of 3 μM stock per hairpin per 400 μl reaction volume). .. We used hairpins containing B2 and B3 adapter sequences with Alexa-488 or Alexa-647 dyes (Molecular Instruments).



Similar Products

93
Biotium tyramide amplification buffer
Tyramide Amplification Buffer, supplied by Biotium, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/amplification+buffer/Tyramide+Amplification+Kit/pm42086546-309-25-28
Average 93 stars, based on 1 article reviews
tyramide amplification buffer - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier

86
Molecular Instruments amplification buffer
Amplification Buffer, supplied by Molecular Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/amplification+buffer/amplification+buffer/10__1096_slash_fj__202601339r-65-10-14
Average 86 stars, based on 1 article reviews
amplification buffer - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier

86
Molecular Instruments amplification buffer 677
Amplification Buffer 677, supplied by Molecular Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/amplification+buffer/amplification+buffer/pm42155877-349-9-12
Average 86 stars, based on 1 article reviews
amplification buffer 677 - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier

86
Molecular Instruments hcr amplification buffer
Hcr Amplification Buffer, supplied by Molecular Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/amplification+buffer/amplification+buffer/bio_rxiv__64898__2026__04__28__719500-609-14-17
Average 86 stars, based on 1 article reviews
hcr amplification buffer - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier

Image Search Results